Totally, there are two modules in CD-NuSS web server: one is to predict the Nucleic acid secondary structural conformation and the other is to determine the dissociation constant corresponding to the biomolecular interactions
In the Nucleic acid secondary structural conformation prediction, a single CD spectral data can be uploaded and analyzed for the secondary structure type prediction of nucleic acids. Data can be uploaded as a text file with two columns, separated by a space, wherein, the X axis contains the wavelength and y axis contains the ellipticity information (Delta epsilon (M ^ -1 cm ^ -1) or ellipticity (mdeg). An example for the CD spectral file has been given below.